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probe oligos  (Lumiprobe)


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    Structured Review

    Lumiprobe probe oligos
    Probe Oligos, supplied by Lumiprobe, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/probe+oligos/Amino-11-ddUTP/pm41413345-155-21-29
    Average 93 stars, based on 41 article reviews
    probe oligos - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    other:

    Article Title: SpaceBar enables single-cell-resolution clone tracing with imaging-based spatial transcriptomics.
    Article Snippet: Imaging-based spatial transcriptomics methods allow for the measurement of spatial determinants of cellular phenotypes but are incompatible with random barcode-based clone-tracing methods, preventing the simultaneous detection of clonal and spatial drivers.. Here we report SpaceBar, which enables simultaneous clone tracing and spatial gene expression profiling with standard imaging-based spatial transcriptomics pipelines.. Our approach uses a library of 96 synthetic barcode sequences that combinatorially labels each cell.



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    a, Schematic of the two-part labeling workflow. Multiple antibodies are photo-crosslinked to the same oYo-Link <t>oligo,</t> then individually hybridized with distinct barcode probes carrying unique L + R barcode pairs. The barcoded antibody complexes are pooled for multiplex immunostaining. b–f, Distinct protein patterns visualized by cycleHCR in a mouse brain section using antibody complexes conjugated with the same oYo-Link oligo. Antibodies from the same host species were used simultaneously without cross-reactivity (host species indicated for each antibody target in the figure: R, rabbit; M, mouse). Orthogonal 3D rendering performed in Imaris. b, Five-color composite showing CREB-binding protein (CBP), neuron-specific βIII-tubulin (Tuj1), parvalbumin (PV), cannabinoid receptor 1 (CB1), somatostatin receptor 2 (Sstr2), and calbindin in the hippocampal region of a mouse brain section. These targets belong to a subset of 11 antibodies crosslinked to the same oYo-Link sequence (No. 2; ) within the complete 46-protein panel. Scale bar, 100 μm. c, (Left) 3D composite of two nuclear proteins, H4K16ac and MECP2, from the same oYo-Link (No. 2) set. (Right) Corresponding DAPI channel of the same nucleus. Scale bars, 5 μm. d, Three-color composite of SF3a66, AQP4, and VGLUT2 from another oYo-Link (No. 1) set (four antibodies) within the 46-target panel. Scale bar, 500 μm. e,f, Higher-magnification views of boxed regions in (b), showing fine subcellular localization and structural detail. Scale bars (e, f), 20 μm.
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    Thermo Fisher biotinylated dna oligo probes
    a, Schematic of the two-part labeling workflow. Multiple antibodies are photo-crosslinked to the same oYo-Link <t>oligo,</t> then individually hybridized with distinct barcode probes carrying unique L + R barcode pairs. The barcoded antibody complexes are pooled for multiplex immunostaining. b–f, Distinct protein patterns visualized by cycleHCR in a mouse brain section using antibody complexes conjugated with the same oYo-Link oligo. Antibodies from the same host species were used simultaneously without cross-reactivity (host species indicated for each antibody target in the figure: R, rabbit; M, mouse). Orthogonal 3D rendering performed in Imaris. b, Five-color composite showing CREB-binding protein (CBP), neuron-specific βIII-tubulin (Tuj1), parvalbumin (PV), cannabinoid receptor 1 (CB1), somatostatin receptor 2 (Sstr2), and calbindin in the hippocampal region of a mouse brain section. These targets belong to a subset of 11 antibodies crosslinked to the same oYo-Link sequence (No. 2; ) within the complete 46-protein panel. Scale bar, 100 μm. c, (Left) 3D composite of two nuclear proteins, H4K16ac and MECP2, from the same oYo-Link (No. 2) set. (Right) Corresponding DAPI channel of the same nucleus. Scale bars, 5 μm. d, Three-color composite of SF3a66, AQP4, and VGLUT2 from another oYo-Link (No. 1) set (four antibodies) within the 46-target panel. Scale bar, 500 μm. e,f, Higher-magnification views of boxed regions in (b), showing fine subcellular localization and structural detail. Scale bars (e, f), 20 μm.
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    Thermo Fisher oligo probes
    a, Schematic of the two-part labeling workflow. Multiple antibodies are photo-crosslinked to the same oYo-Link <t>oligo,</t> then individually hybridized with distinct barcode probes carrying unique L + R barcode pairs. The barcoded antibody complexes are pooled for multiplex immunostaining. b–f, Distinct protein patterns visualized by cycleHCR in a mouse brain section using antibody complexes conjugated with the same oYo-Link oligo. Antibodies from the same host species were used simultaneously without cross-reactivity (host species indicated for each antibody target in the figure: R, rabbit; M, mouse). Orthogonal 3D rendering performed in Imaris. b, Five-color composite showing CREB-binding protein (CBP), neuron-specific βIII-tubulin (Tuj1), parvalbumin (PV), cannabinoid receptor 1 (CB1), somatostatin receptor 2 (Sstr2), and calbindin in the hippocampal region of a mouse brain section. These targets belong to a subset of 11 antibodies crosslinked to the same oYo-Link sequence (No. 2; ) within the complete 46-protein panel. Scale bar, 100 μm. c, (Left) 3D composite of two nuclear proteins, H4K16ac and MECP2, from the same oYo-Link (No. 2) set. (Right) Corresponding DAPI channel of the same nucleus. Scale bars, 5 μm. d, Three-color composite of SF3a66, AQP4, and VGLUT2 from another oYo-Link (No. 1) set (four antibodies) within the 46-target panel. Scale bar, 500 μm. e,f, Higher-magnification views of boxed regions in (b), showing fine subcellular localization and structural detail. Scale bars (e, f), 20 μm.
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    Image Search Results


    a, Schematic of the two-part labeling workflow. Multiple antibodies are photo-crosslinked to the same oYo-Link oligo, then individually hybridized with distinct barcode probes carrying unique L + R barcode pairs. The barcoded antibody complexes are pooled for multiplex immunostaining. b–f, Distinct protein patterns visualized by cycleHCR in a mouse brain section using antibody complexes conjugated with the same oYo-Link oligo. Antibodies from the same host species were used simultaneously without cross-reactivity (host species indicated for each antibody target in the figure: R, rabbit; M, mouse). Orthogonal 3D rendering performed in Imaris. b, Five-color composite showing CREB-binding protein (CBP), neuron-specific βIII-tubulin (Tuj1), parvalbumin (PV), cannabinoid receptor 1 (CB1), somatostatin receptor 2 (Sstr2), and calbindin in the hippocampal region of a mouse brain section. These targets belong to a subset of 11 antibodies crosslinked to the same oYo-Link sequence (No. 2; ) within the complete 46-protein panel. Scale bar, 100 μm. c, (Left) 3D composite of two nuclear proteins, H4K16ac and MECP2, from the same oYo-Link (No. 2) set. (Right) Corresponding DAPI channel of the same nucleus. Scale bars, 5 μm. d, Three-color composite of SF3a66, AQP4, and VGLUT2 from another oYo-Link (No. 1) set (four antibodies) within the 46-target panel. Scale bar, 500 μm. e,f, Higher-magnification views of boxed regions in (b), showing fine subcellular localization and structural detail. Scale bars (e, f), 20 μm.

    Journal: bioRxiv

    Article Title: Machine learning-guided spatial omics for tissue-scale discovery of cell-type-specific architectures

    doi: 10.64898/2026.02.12.705598

    Figure Lengend Snippet: a, Schematic of the two-part labeling workflow. Multiple antibodies are photo-crosslinked to the same oYo-Link oligo, then individually hybridized with distinct barcode probes carrying unique L + R barcode pairs. The barcoded antibody complexes are pooled for multiplex immunostaining. b–f, Distinct protein patterns visualized by cycleHCR in a mouse brain section using antibody complexes conjugated with the same oYo-Link oligo. Antibodies from the same host species were used simultaneously without cross-reactivity (host species indicated for each antibody target in the figure: R, rabbit; M, mouse). Orthogonal 3D rendering performed in Imaris. b, Five-color composite showing CREB-binding protein (CBP), neuron-specific βIII-tubulin (Tuj1), parvalbumin (PV), cannabinoid receptor 1 (CB1), somatostatin receptor 2 (Sstr2), and calbindin in the hippocampal region of a mouse brain section. These targets belong to a subset of 11 antibodies crosslinked to the same oYo-Link sequence (No. 2; ) within the complete 46-protein panel. Scale bar, 100 μm. c, (Left) 3D composite of two nuclear proteins, H4K16ac and MECP2, from the same oYo-Link (No. 2) set. (Right) Corresponding DAPI channel of the same nucleus. Scale bars, 5 μm. d, Three-color composite of SF3a66, AQP4, and VGLUT2 from another oYo-Link (No. 1) set (four antibodies) within the 46-target panel. Scale bar, 500 μm. e,f, Higher-magnification views of boxed regions in (b), showing fine subcellular localization and structural detail. Scale bars (e, f), 20 μm.

    Article Snippet: Briefly, the RNA primary probe oligo library (Twist Bioscience) was amplified by PCR using KAPA HiFi HotStart (Roche, KK2502) with primers containing T7 promoter sequences (IDT).

    Techniques: Labeling, Multiplex Assay, Immunostaining, Binding Assay, Sequencing

    Journal: bioRxiv

    Article Title: Machine learning-guided spatial omics for tissue-scale discovery of cell-type-specific architectures

    doi: 10.64898/2026.02.12.705598

    Figure Lengend Snippet:

    Article Snippet: Briefly, the RNA primary probe oligo library (Twist Bioscience) was amplified by PCR using KAPA HiFi HotStart (Roche, KK2502) with primers containing T7 promoter sequences (IDT).

    Techniques: Imaging, Sequencing, Multiplex Assay, Expressing, Immunostaining